human umbilical vein endothelial cells uvecs  (ATCC)

Journal: Bioactive Materials

Article Title: A bioactive material with dual integrin-targeting ligands regulates specific endogenous cell adhesion and promotes vascularized bone regeneration in adult and fetal bone defects

doi: 10.1016/j.bioactmat.2022.05.027

Figure Lengend Snippet: Adhesion of human BMSCs, osteoblasts, ECFCs and UVECs on the LLP2A/LXW7 modified biomaterials in vitro . a, Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered biomaterials modified with different integrin binding ligands. b , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrin α4β1 expressed on the cells was blocked. c , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrin αvβ3 expressed on the cells was blocked. d , Quantitation of total DNA of human BMSCs, osteoblasts, ECFCs and UVECs adhered on biomaterials modified with different integrin binding ligands after integrins α4β1 and αvβ3 expressed on the cells were blocked. Data were expressed as mean ± standard deviation: **p < 0.01, ***p < 0.001 (n = 6).

Article Snippet: Human umbilical vein endothelial cells (UVECs) were purchased from ATCC (PCS-100-013) and expanded in Endothelial Cell Growth Medium-2 (EGM-2, CC-3162, Lonza).

Techniques: Modification, In Vitro, Quantitation Assay, Binding Assay, Standard Deviation

Journal: Journal of Tissue Engineering

Article Title: Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects

doi: 10.1177/20417314241306681

Figure Lengend Snippet: Toxic effects of GOG@WK-EVs on cells. (a) Co-cultured materials with various groups of cells for 6, 12, 24, and 48 h and evaluated the cell status and viability of BMSCs, UVECs, and BMMs using live/dead cell staining and MTT assay; (b) Assessed cell survival and proliferation of BMSCs, UVECs, and BMMs using CCK-8 assay; (c) Examined the cytoskeletal structure of BMSCs, UVECs, and BMMs through immunofluorescence staining, where phalloidin staining indicated cytoskeleton in green, DAPI staining showed nuclei in blue, scale bar: 50 μm. * denotes statistical significance at p < 0.05 between two groups and the cell experiments were repeated three times.

Article Snippet: Rat umbilical vein endothelial cells (UVECs) were obtained from Procell (Catalog Number: CP-R232) and cultured in endothelial cell medium (ECM, Catalog Number: 1001, Wegene, China) supplemented with 5% fetal bovine serum, 1% v/v penicillin/streptomycin, and 1% ECGS (Catalog Number: KGY1052, KGI, China) at 37°C in a 5% CO 2 environment.

Techniques: Cell Culture, Staining, MTT Assay, CCK-8 Assay, Immunofluorescence

Journal: Journal of Tissue Engineering

Article Title: Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects

doi: 10.1177/20417314241306681

Figure Lengend Snippet: Impact of GOG@WK-EVs on vascular formation in UVECs. (a) Transwell assay to evaluate the migration of UVECs in each group, scale bar: 50 μm; (b) Scratch assay to measure the migration distance of UVECs in each group, scale bar: 100 μm; (c) Observation of the vascular formation capacity of UVECs in each group under an optical microscope, scale bar: 100 μm; (d) Number of branch points formed by UVECs in each group as depicted in Figure C; (E) Total length of tubes formed by UVECs in each group as depicted in Figure c; (f) Gene expression levels of key vascular formation factors (Ang, Pecam1, and Vcam1) in UVECs in each group assessed by RT-qPCR. * indicates a statistically significant difference ( p < 0.05) between the two groups, with cell experiments repeated three times.

Article Snippet: Rat umbilical vein endothelial cells (UVECs) were obtained from Procell (Catalog Number: CP-R232) and cultured in endothelial cell medium (ECM, Catalog Number: 1001, Wegene, China) supplemented with 5% fetal bovine serum, 1% v/v penicillin/streptomycin, and 1% ECGS (Catalog Number: KGY1052, KGI, China) at 37°C in a 5% CO 2 environment.

Techniques: Transwell Assay, Migration, Wound Healing Assay, Microscopy, Gene Expression, Quantitative RT-PCR